Cd- and Zn-NagA ended up utilised to address the incidence of direct interactions from the carbonyl oxygen/sulfur as well as metal ion via a comparison from the kinetic constants for N

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b will be the dissociation constant with the team that should be protonated for comprehensive action. The pH profiles which confirmed the deprotonation of two acid ic teams and also the protonation of 1 standard group are demanded for optimum action were match to equation five where K

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m with compound daftar naga26 one is due to protonation of the phosphate moiety to the mono-anion. Additionally it is curious to notice which the ionization that is definitely observed at significant pH within the log k